Recombinant hTASK1 Is an O2-Sensitive K+ Channel

Hypoxic inhibition of background K+ channels is crucial to O2 sensing by chemoreceptor tissues, but direct demonstration of O2 sensitivity by any member of this K+ channel family is lacking. HEK293 cells were transfected with a pcDNA3.1-hTASK1 construct; expression of hTASK1 was verified using RT-PC...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-08, Vol.285 (5), p.1290-1294
Main Authors: Lewis, A., Hartness, M.E., Chapman, C.G., Fearon, I.M., Meadows, H.J., Peers, C., Kemp, P.J.
Format: Article
Language:English
Subjects:
hypoxia
O2 sensing
pH sensing
tandem P domain K+ channel
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Summary:Hypoxic inhibition of background K+ channels is crucial to O2 sensing by chemoreceptor tissues, but direct demonstration of O2 sensitivity by any member of this K+ channel family is lacking. HEK293 cells were transfected with a pcDNA3.1-hTASK1 construct; expression of hTASK1 was verified using RT-PCR and immunocytochemistry. Whole-cell K+ currents of cells stably expressing hTASK-1 were, as anticipated, extremely sensitive to extracellular pH, within the physiological range (IC50 ≈ 7.0). All cells expressing this signature pH sensitivity were acutely modulated by pO2; reduction of pO2 from 150 to
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5310