Analysis of Ca2+ Currents in Spermatocytes from Mice Lacking Cav2.3 (α1E) Ca2+ Channel

In mammalian male germ-line cells, low-voltage-activated (LVA) Ca2+ current has been identified and its electrophysiological properties have been studied. To investigate whether α12.3 (α1E) subunit of the voltage-dependent Ca2+ channel codes for the LVA current, whole-cell patch clamp and following...

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Published in:Biochemical and biophysical research communications 2001-11, Vol.288 (4), p.1032-1036
Main Authors: Sakata, Yu, Saegusa, Hironao, Zong, Shuqin, Osanai, Makoto, Murakoshi, Takayuki, Shimizu, Yasufumi, Noda, Tetsuo, Aso, Takeshi, Tanabe, Tsutomu
Format: Article
Language:English
Subjects:
Cav2.3
Cav3.1
knockout mouse
low-voltage-activated Ca2+ currents
pachytene spermatocyte
R-type
T-type
voltage-dependent Ca2+ channel
α1E
α1G
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Summary:In mammalian male germ-line cells, low-voltage-activated (LVA) Ca2+ current has been identified and its electrophysiological properties have been studied. To investigate whether α12.3 (α1E) subunit of the voltage-dependent Ca2+ channel codes for the LVA current, whole-cell patch clamp and following reverse transcription-polymerase chain reaction (RT-PCR) experiments were performed in pachytene spermatocytes from Cav2.3+/+ and Cav2.3−/− mice. Whole-cell current in acutely dissociated pachytene spermatocytes from Cav2.3+/+ and Cav2.3−/− mice displayed a typical profile of LVA Ca2+ currents and kinetics with no significant differences. Single-cell RT-PCR revealed the expression of Cacna1g in the pachytene spermatocytes from Cav2.3+/+ and Cav2.3−/− mice in which LVA Ca2+ currents were actually recorded. These results suggest that the Cav2.3 channel makes no detectable contribution to the LVA Ca2+ current in the pachytene spermatocyte. Instead, Cav3 family such as Cav3.1 may be the likely candidates responsible for the LVA currents in pachytene spermatocytes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5871