RNA RELOCATION AT MITOSIS IN TRANSFORMED AND TUMORIGENIC HUMAN BREAST EPITHELIAL CELLS

The fate of RNA revealed by metachromatic staining after a critical electrolyte concentration assay using toluidine blue and Mg2+ions as competitors for the substrate dye binding sites was followed at mitosis in human breast epithelial cells transformed by benzo[a]pyrene and transfected with the c-H...

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Bibliographic Details
Published in:Cell biology international 1999-02, Vol.23 (2), p.125-128
Main Authors: Mello, Maria Luiza S, Barbisan, Luis F, Russo, Jose, Vidal, Benedicto De C
Format: Article
Language:English
Subjects:
Benzo(a)pyrene - toxicity
Biological Transport, Active
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line, Transformed
Cell Nucleolus - pathology
cytochemistry
Epithelial Cells - metabolism
Epithelial Cells - pathology
Female
Genes, ras
Humans
Interphase
Mitosis
nucleolus-like bodies
RNA
RNA, Neoplasm - metabolism
transformation
Tumor Cells, Cultured
tumorigenesis
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Summary:The fate of RNA revealed by metachromatic staining after a critical electrolyte concentration assay using toluidine blue and Mg2+ions as competitors for the substrate dye binding sites was followed at mitosis in human breast epithelial cells transformed by benzo[a]pyrene and transfected with the c-Ha- ras oncogene. The aim was to detect changes in RNA distribution during mitosis in human transformed/tumorigenic cells exhibiting increased nucleolar sizes and rRNA production while in interphase. RNA relocation in association with the mitotic spindle fibers was observed from metaphase to telophase not to vary in all the cell lines studied. RNA-containing nucleolus-like bodies persistent during mitosis were found to decrease in frequency in the transformed and tumorigenic cells in comparison with control non-transformed cells simultaneously to the previously reported increase in nucleolar areas for the same cells while in interphase. It is suggested that an improved use of RNA transcripts has been developed with cell transformation and tumorigenesis in this particular model.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.1998.0340