DIFFERENCES IN RAT RBC CYTOSOL INDUCED AFTER IN VIVO PHENYLHYDRAZINE TREATMENT

Analysis of rat red blood cell (RBC) cytosol protein fraction after in vivo phenylhydrazine treatment revealed increased amounts of 68-kDa protein. This protein is present in trace amounts in normal rat RBC cytosol. We also present data that 68-kDa protein from RBC cytosol has an identical isoelectr...

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Bibliographic Details
Published in:Cell biology international 1999-10, Vol.23 (10), p.677-683
Main Authors: Djurkovic, Aleksandra, Zaric, Jelena, Lušic, Marina, Glišin, Vladimir, Popovic, Zvezdana
Format: Article
Language:English
Subjects:
Anemia, Hemolytic - chemically induced
Anemia, Hemolytic - metabolism
Animals
Blood Proteins - analysis
Blood Proteins - metabolism
Blotting, Western
Cytosol - chemistry
Cytosol - drug effects
Electrophoresis, Gel, Two-Dimensional
Erythrocytes - drug effects
Exocytosis - drug effects
haemolytic anaemia
Peptide Mapping
Phenylhydrazines - pharmacology
PHZ treatment
rat RBC cytosol
Rats
Rats, Wistar
Serine Endopeptidases - pharmacology
Trypsin - pharmacology
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Summary:Analysis of rat red blood cell (RBC) cytosol protein fraction after in vivo phenylhydrazine treatment revealed increased amounts of 68-kDa protein. This protein is present in trace amounts in normal rat RBC cytosol. We also present data that 68-kDa protein from RBC cytosol has an identical isoelectric point, partial proteolytic map and immunological determinants as a protein of the same molecular mass from rat exosomes. These data indicate that 68-kDa protein is normally present in rat RBC cytosol, is exported via exosomes during reticulocyte maturation, and is increased in induced haemolytic anaemia.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.1999.0434