INHIBITION OF CYTOKINESIS BY ASBESTOS AND SYNTHETIC FIBRES

Using high-resolution timelapse microscopy, we have followed individual phagocytized fibres through the later stages of division in MeT-5A human mesothelial cells and LLC-MK2monkey epithelial cells. The fibres used were crocidolite and chrysotile asbestos, fibrous glass (MMVF), and refractory cerami...

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Bibliographic Details
Published in:Cell biology international 1999-12, Vol.23 (12), p.829-840
Main Authors: Jensen, Cynthia G, Watson, Maureen
Format: Article
Language:English
Subjects:
Animals
asbestos
Asbestos - toxicity
binucleate
Cell Division - drug effects
Cell Line
Cell Nucleus - drug effects
Cell Nucleus - pathology
Cell Nucleus - ultrastructure
Ceramics - toxicity
cytokinesis
fibre
Glass
Humans
Microscopy, Electron
Microscopy, Fluorescence
Microscopy, Video
Mineral Fibers - toxicity
Mitosis - drug effects
MMVF
Phagocytosis
RCF
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Summary:Using high-resolution timelapse microscopy, we have followed individual phagocytized fibres through the later stages of division in MeT-5A human mesothelial cells and LLC-MK2monkey epithelial cells. The fibres used were crocidolite and chrysotile asbestos, fibrous glass (MMVF), and refractory ceramic fibres (RCF). Long fibres (15–80μm) trapped within the cleavage furrow can partially or completely block cytokinesis. Cells proceed in one of three ways: (1) eventual completion of cytokinesis; (2) incomplete cytokinesis, resulting in two cells joined by a fibre-containing intercellular channel; or (3) failure of cytokinesis, resulting in a binucleate or trinucleate cell. Two factors associated with fibre-induced bi/trinucleation are: (1) an initial association between the fibre and the forming daughter nuclei, which is sometimes lost over time, and (2) disintegration of the midbody. The studies suggest that delay of cytokinesis by interzonal fibres can result in bi/trinucleation through the loss of midbody/intercellular bridge proteins that are required for completion of cytokinesis.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.1999.0479