APOPTOTIC AND NON-APOPTOTIC MODES OF PROGRAMMED CELL DEATH IN MCF-7 HUMAN BREAST CARCINOMA CELLS

Apoptosis is a specific mode of programmed cell death (PCD), recognized by characteristic morphological and molecular changes. Here we present evidence for a non-apoptotic type of PCD in human MCF-7 breast carcinoma cells. We used TNF-α and tyrphostin AG213 to induce apoptotic and non-apoptotic cell...

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Bibliographic Details
Published in:Cell biology international 2000-04, Vol.24 (4), p.253-260
Main Authors: Amin, Farhana, Bowen, Ivor D., Szegedi, Zsolt, Mihalik, Rudolf, Szende, Bela
Format: Article
Language:English
Subjects:
Apoptosis
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Nucleus - ultrastructure
Comet Assay
Cytoplasm - ultrastructure
DNA Fragmentation
DNA, Neoplasm - analysis
Female
Humans
MFC-7 breast carcinoma
Microscopy, Electron, Scanning
Microvilli - ultrastructure
non-apoptotic programmed cell death
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Pseudopodia - ultrastructure
RNA, Neoplasm - analysis
TNF-α
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - pharmacology
Tumor Suppressor Protein p53 - biosynthesis
tyrphostin AG213
Tyrphostins - pharmacology
Vacuoles - ultrastructure
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Summary:Apoptosis is a specific mode of programmed cell death (PCD), recognized by characteristic morphological and molecular changes. Here we present evidence for a non-apoptotic type of PCD in human MCF-7 breast carcinoma cells. We used TNF-α and tyrphostin AG213 to induce apoptotic and non-apoptotic cell death respectively in vitro. Microscopic and immunohistochemical studies, together with DNA analysis and flow cytometric analysis of p53 and bcl-2 oncogene expression, revealed some novel characteristics of non-apoptotic cell death. We show here for the first time some of the biochemical features of an experimentally induced non-apoptotic PCD and emphasize the distinct biochemical events leading to apoptotic and non-apoptotic PCD.
ISSN:1065-6995
1095-8355
DOI:10.1006/cbir.2000.0495