Vγ9 -Vδ2 + γδ T Cells from a Patient with Felty Syndrome That Exhibit Aberrant Response to Triggering of the CD3 Molecule Can Regulate Immunoglobulin Secretion by B Cells

• Published in: Clinical immunology and immunopathology 1995, Vol.74 (2), p.162-169
• Main Authors: Bank, Ilan, Tanay, Amir, Migdal, Amiel, Book, Mazal, Livneh, Avi
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Antibody production; Biological and medical sciences; Diseases of the osteoarticular system; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Inflammatory joint diseases; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Medical sciences
• ISSN: 0090-1229; 1090-2341
• DOI: 10.1006/clin.1995.1024

Expanded populations of T lymphocytes bearing γδ T cell receptors have been detected in several patients with Felty syndrome. The goal of this study was to functionally characterize these lymphocytes in a newly described patient with this disease. For this, fluorescence-activated cell sorter analysis of T cell surface antigens, proliferation, and tumor necrosis factor α enzyme-linked immunosorbent assays, as well as quantitative assays of immunoglobulins secreted by pokeweed mitogen-driven B cells were performed. The γδ cells, that expressed a CD3 +CD4 -Vγ9 -Vδ2 +Cδ + phenotype, and constituted 60% of the peripheral blood T cells, did not proliferate after triggering with anti-CD3, but did secrete tumor necrosis factor α and the addition of these cells to pokeweed mitogen-stimulated B cells from the patient decreased their secretion of immunoglobulin M while augmenting IgG secretion. These data suggest that the expanded anergic Vγ9 -Vδ2 + γδ cells can play an immunoregulatory role in the patient.