Improvement of Motility and Fertilization Potential of Postthaw Human Sperm Using Glutamine

The effectiveness of three amino acids, glutamine, proline, and histidine, and one amino acid-related compound, betaine, in preserving human sperm diluted v/v in a basal medium (BM) containing 14% glycerol during the freeze–thaw (FT) process was studied. At 80 mMin BM, only glutamine improved the 5-...

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Bibliographic Details
Published in:Cryobiology 1996-06, Vol.33 (3), p.311-319
Main Authors: RENARD, PHILIPPE, GRIZARD, GENEVIEVE, GRIVEAU, JEAN-FRANCOIS, SION, BENOIT, BOUCHER, DANIEL, LE LANNOU, DOMINIQUE
Format: Article
Language:English
Subjects:
Animals
Betaine
Biological and medical sciences
Birth control
Cricetinae
Cryopreservation - methods
Cryoprotective Agents
Evaluation Studies as Topic
Female
Fertilization in Vitro
Glutamine
Glycerol
Gynecology. Andrology. Obstetrics
Histidine
Humans
In Vitro Techniques
Male
Medical sciences
Mesocricetus
Proline
Solutions
Sperm Motility
Sperm-Ovum Interactions
Spermatozoa
Sterility. Assisted procreation
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Summary:The effectiveness of three amino acids, glutamine, proline, and histidine, and one amino acid-related compound, betaine, in preserving human sperm diluted v/v in a basal medium (BM) containing 14% glycerol during the freeze–thaw (FT) process was studied. At 80 mMin BM, only glutamine improved the 5- to 60-min postthaw total and progressive motilities and velocity. The presence of glutamine at 80 mMis not sufficient to achieve lower concentrations of the toxic agent glycerol in FT medium. Glutamine may therefore have a mechanism of protection on human spermatozoa that is independent from that of glycerol. The zona-free hamster egg penetration test showed that the percentage of eggs penetrated was significantly greater when spermatozoa were frozen–thawed with 80 mMglutamine in BM. Consequently, the presence of glutamine at 80 mMin a glycerol–FT medium maintains human sperm motility and fertilizing ability during the FT process.
ISSN:0011-2240
1090-2392
DOI:10.1006/cryo.1996.0031