LIPOPOLYSACCHARIDE/ENDOTOXIN INDUCES IL-18 VIA CD14 IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS IN VITRO

IL-18 shares activities with IL-12 in generating T-helper 1 cells and cytokine response. It mediates LPS/endotoxin lethality by IL-12 independent interferon-γ synthesis and it induces bacteria-related organ failure. As peripheral blood mononuclear cells (PBMC) are potent producers of IL-18, we studi...

Full description

Saved in:
Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2000-12, Vol.12 (12), p.1788-1792
Main Authors: Manigold, Tobias, Böcker, Ulrich, Traber, Petra, Dong-Si, Tuan, Kurimoto, Masashi, Hanck, Christoph, Singer, Manfred V., Rossol, Siegbert
Format: Article
Language:English
Subjects:
Adult
Dose-Response Relationship, Drug
Endotoxins - pharmacology
Enzyme-Linked Immunosorbent Assay
Humans
IL-18/endotoxin/CD14/PBMC
Interferon-gamma - metabolism
Interleukin-12 - metabolism
Interleukin-18 - metabolism
Leukocytes, Mononuclear - metabolism
Lipopolysaccharide Receptors - metabolism
Lipopolysaccharides - pharmacology
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
Staphylococcus aureus - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:IL-18 shares activities with IL-12 in generating T-helper 1 cells and cytokine response. It mediates LPS/endotoxin lethality by IL-12 independent interferon-γ synthesis and it induces bacteria-related organ failure. As peripheral blood mononuclear cells (PBMC) are potent producers of IL-18, we studied the regulation of IL-18 upon exposure to LPS and Staphylococcus aureus (SAC) in vitro. Freshly isolated PBMC constitutively expressed IL-18 mRNA. After unstimulated preincubation for 48h, however, IL-18 transcripts were nearly not detectable by RT-PCR, but inducible by LPS or SAC (P
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.2000.0783