Subcellular Localization of the Small GTPase Rab5a in Resting and Stimulated Human Neutrophils

The evidence that small GTPases of the Rab family are regulators of vesicle traffic which can influence various cell functions prompted us to investigate the potential role of one of these proteins, Rab5a, in human neutrophils. In this paper we show that a large amount of Rab5a is present in the cyt...

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Bibliographic Details
Published in:Experimental cell research 1996-09, Vol.227 (2), p.367-373
Main Authors: Vita, Francesca, Soranzo, Maria Rosa, Borelli, Violetta, Bertoncin, Paolo, Zabucchi, Giuliano
Format: Article
Language:English
Subjects:
Cytoplasmic Granules - chemistry
Cytoplasmic Granules - enzymology
Cytosol - chemistry
Cytosol - enzymology
GTP Phosphohydrolases - analysis
GTP Phosphohydrolases - drug effects
GTP Phosphohydrolases - metabolism
GTP-Binding Proteins - analysis
GTP-Binding Proteins - drug effects
GTP-Binding Proteins - metabolism
Humans
Lymphocyte Activation - physiology
Membrane Proteins - analysis
Microscopy, Electron
Neutrophils - chemistry
Neutrophils - enzymology
Neutrophils - ultrastructure
rab5 GTP-Binding Proteins
Subcellular Fractions - chemistry
Subcellular Fractions - enzymology
Subcellular Fractions - ultrastructure
Tetradecanoylphorbol Acetate - pharmacology
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Summary:The evidence that small GTPases of the Rab family are regulators of vesicle traffic which can influence various cell functions prompted us to investigate the potential role of one of these proteins, Rab5a, in human neutrophils. In this paper we show that a large amount of Rab5a is present in the cytosol of peripheral blood mature neutrophils. The remaining protein was found to be membrane and azurophilic granule associated. Upon neutrophil challenge with PMA for 10 min the amount of membrane-associated Rab5a was upregulated while the cytosolic content of the protein concomitantly decreased. These findings support the hypothesis that Rab5a could be involved in the mechanism of neutrophil activation by modulating the rate of endocytosis and/or vesicle fusion.
ISSN:0014-4827
1090-2422
DOI:10.1006/excr.1996.0286