Inhibition of Glutamate Uptake Causes an Acute Increase in Aqueous Humor Protein

Inhibition of glutamate transport has been shown to increase paracellular permeability of epithelial cell monolayersin vitro. To determine if blocking glutamate transport would affect tissue permeabilityin vivo,D-aspartate (D-Asp; 300 nmol 30 μl−1) (a non-toxic competitive inhibitor of glutamate tra...

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Bibliographic Details
Published in:Experimental eye research 1997-02, Vol.64 (2), p.157-165
Main Authors: LANGFORD, MARLYN P., BERG, M.ELLEN, MACK, JOANN H., GANLEY, JAMES P., WELBOURNE, TOMAS C.
Format: Article
Language:English
Subjects:
Alanine - metabolism
amino acids
Animals
aqueous humor
Aqueous Humor - drug effects
Aqueous Humor - metabolism
Aspartic Acid - analogs & derivatives
Aspartic Acid - metabolism
Aspartic Acid - pharmacology
Biological and medical sciences
Biological Transport - drug effects
blood-aqueous barrier
Cell Membrane Permeability - drug effects
Excitatory Amino Acid Antagonists - pharmacology
eye
Eye and associated structures. Visual pathways and centers. Vision
Eye Proteins - analysis
Eye Proteins - metabolism
Female
Fundamental and applied biological sciences. Psychology
glutamate
Glutamic Acid - metabolism
Glutamine - metabolism
Male
ocular physiology
paracellular permeability
protein
Rabbits
Vertebrates: nervous system and sense organs
γ-glutamyl transferase
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Summary:Inhibition of glutamate transport has been shown to increase paracellular permeability of epithelial cell monolayersin vitro. To determine if blocking glutamate transport would affect tissue permeabilityin vivo,D-aspartate (D-Asp; 300 nmol 30 μl−1) (a non-toxic competitive inhibitor of glutamate transport) or a placebo was injected into the anterior chambers of the fellow eyes of 15 adult rabbits. [14C]-L-glucose and/or [125I]-rabbit albumin were included in the injection vehicle as aqueous humor (AH) outflow markers. The specific inhibition of glutamate uptake byD-Asp was indicated by a 15% increase in AH glutamate (174±9 nmol ml−1to 205±13 nmol ml−1;P=0.03) at 1–1.5 hr post injection. Also, the efflux of [14C]-L-glucose and [125I]-rabbit albumin from the AH of D-Asp injected eyes was increased 22% over the placebo-injected control eyes (P≤0.02). Concomitantly, the total protein concentration in the AH fromD-Asp injected eyes (517±35 μg ml−1) was 19% greater (P
ISSN:0014-4835
1096-0007
DOI:10.1006/exer.1996.0180