Localization ofHuC(ELAVL3) to Chromosome 19p13.2 by Fluorescencein SituHybridization Utilizing a Novel Tyramide Labeling Technique

HuC is a neural-specific member of the Elav family of RNA-binding proteins. This highly conserved gene family plays a crucial role in neurogenesis, andHuC(HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescencein situhybridization (T-FIS...

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Published in:Genomics (San Diego, Calif.) Calif.), 1998-11, Vol.53 (3), p.296-299
Main Authors: Van Tine, Brian A., Knops, Judith F., Butler, Adam, Deloukas, Panos, Shaw, George M., King, Peter H.
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cited_by cdi_FETCH-LOGICAL-c1338-714cf9359e9b8a5e3bf175e5a3d1fb92f733b65b41bc090bb6068b9eb947afc43
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container_title Genomics (San Diego, Calif.)
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creator Van Tine, Brian A.
Knops, Judith F.
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description HuC is a neural-specific member of the Elav family of RNA-binding proteins. This highly conserved gene family plays a crucial role in neurogenesis, andHuC(HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescencein situhybridization (T-FISH) technique, we localizedHuCto chromosome 19p13.2. This localization was confirmed by radiation hybrid mapping and coincides with that ofHuR(HGMW-approved symbol ELAVL1), anotherelavfamily member. Dual T-FISH analysis withHuCandHuRprobes, however, indicated distinct loci, withHuCbeing centromeric to HuR. This study demonstrates the utility of T-FISH in colocalizing two genes on the same chromosomal preparation using only biotinylated probes.
doi_str_mv 10.1006/geno.1998.5468
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title Localization ofHuC(ELAVL3) to Chromosome 19p13.2 by Fluorescencein SituHybridization Utilizing a Novel Tyramide Labeling Technique
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