Amy2Polymorphism as a Possible Marker of β-Glucanase Activity in Barley ( Hordeum vulgareL.)

The polymorphism of barley alpha-amylases, as revealed by isoelectric-focusing (IEF), was studied in a population of 126 doubled-haploids from a cross between the cultivars Morex and Steptoe. In particular, the pattern was determined for an additional low p I alpha-amylase isozyme detected in cultiv...

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Bibliographic Details
Published in:Journal of cereal science 1996-07, Vol.24 (1), p.55-63
Main Authors: Zwickert-Menteur, S., Jestin, L., Branlard, G.
Format: Article
Language:English
Subjects:
alpha-amylase IEF
barley malt
QTL
β-glucanase activity
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Summary:The polymorphism of barley alpha-amylases, as revealed by isoelectric-focusing (IEF), was studied in a population of 126 doubled-haploids from a cross between the cultivars Morex and Steptoe. In particular, the pattern was determined for an additional low p I alpha-amylase isozyme detected in cultivar Steptoe. No significant effect of this isozyme on alpha-amylase activity was observed, but a significant effect on β-glucanase activity was detected for two out of four trial locations. Quantitative Trait Loci (QTL) for alpha-amylase and β-glucanase activities were also investigated. The results suggest a potential location for the β-glucanase locus Glb2, on chromosome 1 close to the Amy2locus for alpha-amylase genes.
ISSN:0733-5210
1095-9963
DOI:10.1006/jcrs.1996.0037