Large-Scale Screening of Cyanogenic Potential in Cassava Roots Using the Enzyme-Based Dipsticks

A protocol was developed to use the enzyme-based dipsticks for large scale screening of cyanogenic potential in cassava roots. It involved direct analysis of root tissue slices. Cyanogenic potential (expressed as mg HCN/kg root fresh weight) of 40 cassava varieties were determined. They ranged from...

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Bibliographic Details
Published in:Journal of food composition and analysis 1998-03, Vol.11 (1), p.2-10
Main Authors: Yeoh, Hock-Hin, Sanchez, Teresa, Iglesias, Carlos A.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
CASSAVA
COMPARISONS
CULTIVARS
CYANOGENESIS
DETERMINATION
FOOD ANALYSIS
Food industries
FOOD SAFETY
FOODS
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
MEASUREMENT
METABOLISM
PROCESSING
QUANTITATIVE ANALYSIS
SAMPLING
SPECTROMETRY
SPECTROPHOTOMETRY
Starch and starchy product industries
TESTING
TOXIC SUBSTANCES
Varietal selection. Specialized plant breeding, plant breeding aims
VARIETIES
Yield, quality, earliness, varia
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Summary:A protocol was developed to use the enzyme-based dipsticks for large scale screening of cyanogenic potential in cassava roots. It involved direct analysis of root tissue slices. Cyanogenic potential (expressed as mg HCN/kg root fresh weight) of 40 cassava varieties were determined. They ranged from about 2 to 590 mg HCN/kg root. Twenty-seven varieties gave values that were comparable to those of control (using root extract). Some varieties were underestimated. For these varieties, values similar to control were obtainable by simply crushing the root slices. Determination of cyanogenic potential based on five roots per variety compared well with those using composite root extracts prepared from three roots (r= 0.81). Semiquantitative estimation of cyanogenic potential for 122 cassava varieties was also carried out by comparing the color of picrate paper against those of known standards; results showed a strong correlation with spectrophotometric method (r= 0.79).
ISSN:0889-1575
1096-0481
DOI:10.1006/jfca.1997.0555