A Comparison of Three Commercial Perfluorocarbon Emulsions as High-Field 19F NMR Probes of Oxygen Tension and Temperature

• Published in: Journal of magnetic resonance. Series B 1995-02, Vol.106 (2), p.131-141
• Main Authors: Shukla, H.P., Mason, R.P., Woessner, D.E., Antich, P.P.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cardiovascular system; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Radiodiagnosis. Nmr imagery. Nmr spectrometry
• ISSN: 1064-1866; 1096-0872
• DOI: 10.1006/jmrb.1995.1023

The increasing use of perfluorocarbons (PFCs) as probes of tissue oxygenation on the basis of the 19F spin-lattice relaxation rate ( R 1) prompts comparison between several commercially available PFC blood-substitute emulsions, The relative sensitivities to both oxygen and temperature of the emulsions Oxypherol, Oxygent, and Fluosol at 7 T have been investigated, The linear response of R 1 with pO 2 was confirmed for each resonance in the range 4-50°C, In general, R 1 is not a linear function of temperature, and we demonstrate this for perfluorotributylamine (FTBA) over an extended range of temperatures, 239-343 K. Theoretical R 1 values of FTBA were compared with experimental data by computer modeling of relaxation mechanisms, To further understand the structure-function relationships of PFCs, 2D COSY experiments were used for the assignment of resonances and as an aid in interpretation of relaxation mechanisms, Finally, the utility of Oxygent and Oxypherol for measuring tissue pO 2 in perfused rat hearts was compared before, during, and after eight minutes of global ischemia.