Cytochrome P450 2E1 mRNA in the rat prostate: detection and quantitation by competitive reverse transcription and polymerase chain reaction

Cytochrome P450 2E1 plays a pivotal role in the metabolic activation of a wide variety of low molecular weight environmental toxicants and procarcinogens. In the present study, expression of the P450 2E1 gene in the rat prostate gland was quantitated by competitive reverse transcription and the poly...

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Bibliographic Details
Published in:Molecular and cellular probes 1998-10, Vol.12 (5), p.263-271
Main Authors: Jiang, Y, Kuo, C.-L, Pernecky, S.J, Coon, M.J, Piper, W.N
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cytochrome P-450 CYP2E1 - genetics
cytochrome P450 2E1, gene expression, prostate, polymerase chain reaction (PCR), pyridine, procarcinogens
DNA Primers
DNA, Complementary
Male
Molecular Sequence Data
Prostate - enzymology
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction - instrumentation
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - analysis
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Summary:Cytochrome P450 2E1 plays a pivotal role in the metabolic activation of a wide variety of low molecular weight environmental toxicants and procarcinogens. In the present study, expression of the P450 2E1 gene in the rat prostate gland was quantitated by competitive reverse transcription and the polymerase chain reaction. To assess accurately the induction level of P450 2E1 mRNA in the prostate after pyridine treatment of rats, a recombinant standard RNA was generated that is homologous to the sequence of P450 2E1 mRNA except for an internal deletion of 100 bases. The data indicate that P450 2E1 mRNA is present in the prostate of untreated animals and is induced about four-fold by treatment with pyridine. The results suggest that exposure to certain environmental chemicals and procarcinogens may increase P450 2E1 levels in the prostate gland and thus could enhance formation of reactive, carcinogenic metabolites.
ISSN:0890-8508
1096-1194
DOI:10.1006/mcpr.1998.0177