Neurotransmitter Release from Semi-intact Synaptosomes

We have developed a secretion assay composed of semi-intact synaptosomes from which transmitter release is optimally evoked by micromolar Ca2+in the presence of cytosol. Transmitter release from this preparation reconstitutes known characteristics of regulated exocytosis and is accompanied by a mark...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 1998-10, Vol.16 (2), p.198-203
Main Authors: Tandon, A., Tan, P.K., Bannykh, S., Banerjee, A., Balch, W.E.
Format: Article
Language:English
Subjects:
Animals
Biological Transport - drug effects
Botulinum Toxins - pharmacology
Calcium - metabolism
Cerebral Cortex - metabolism
Cytosol - metabolism
Exocytosis
Glutamates - secretion
Neurotransmitter Agents - secretion
Norepinephrine - secretion
Rats
Synaptosomes - metabolism
Synaptosomes - ultrastructure
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Summary:We have developed a secretion assay composed of semi-intact synaptosomes from which transmitter release is optimally evoked by micromolar Ca2+in the presence of cytosol. Transmitter release from this preparation reconstitutes known characteristics of regulated exocytosis and is accompanied by a marked decrease in synaptic vesicles. The assay is useful in characterizing the components known to be involved in transmitter release, and should also facilitate the identification of additional factors that are important for this process.
ISSN:1046-2023
1095-9130
DOI:10.1006/meth.1998.0667