Expression of Membrane-Bound and Soluble Cell Adhesion Molecules by Human Brain Microvessel Endothelial Cells

• Published in: Microvascular research 1999-01, Vol.57 (1), p.52-60
• Main Authors: Vastag, Monika, Skopál, Judit, Voko, Zoltán, Csonka, Éva, Nagy, Zoltán
• Format: Article
• Language: English
• Subjects: adhesion molecules; Biological and medical sciences; Blood vessels and receptors; Brain - blood supply; Capillaries - drug effects; Capillaries - metabolism; Cells, Cultured; Drug Interactions; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; human brain microvessel endothelium; Humans; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Neuroprotective Agents - pharmacology; piracetam; Piracetam - pharmacology; Solubility; tumor necrosis factor; Tumor Necrosis Factor-alpha - pharmacology; Vascular Cell Adhesion Molecule-1 - biosynthesis; Vascular Cell Adhesion Molecule-1 - genetics; Vertebrates: cardiovascular system
• ISSN: 0026-2862; 1095-9319
• DOI: 10.1006/mvre.1998.2115

Expression of membrane-bound (mb) and soluble (s) forms of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) induced by tumor necrosis factor-α (TNF-α) has been measured by enzyme-linked immunosorbent assay in cultured human brain microvessel endothelial cells. Both the mb and the s forms of VCAM-1 and ICAM-1 were upregulated by TNF-α; however, the stimulation of the s forms was delayed in time. When piracetam, a neuroprotective drug, was added to the tissue culture medium simultaneously with TNF-α, the expression of mbVCAM-1 and ICAM-1 was lowered. Differential upregulation of mb and s forms of adhesion molecules and a novel effect of piracetam have been demonstrated in human brain microvessel endothelial cell cultures.