The Survival of Skeletal Muscle Myoblasts in Vitro Is Sensitive to a Donor of Nitric Oxide and Superoxide, SIN-1, but Not to Nitric Oxide or Peroxynitrite Alone

• Published in: Nitric oxide 1999-08, Vol.3 (4), p.273-280
• Main Authors: Lepore, D.A., Stewart, A.G., Tomasi, A., Anderson, R.L., Hurley, J.V., Morrison, W.A.
• Format: Article
• Language: English
• Subjects: Animals; Cell Line - drug effects; Cell Survival - drug effects; Molsidomine - analogs & derivatives; Molsidomine - pharmacology; Molsidomine - toxicity; Muscle, Skeletal - cytology; Muscle, Skeletal - drug effects; myoblasts; Nitrates - pharmacology; Nitrates - toxicity; nitric oxide; Nitric Oxide - pharmacology; Nitric Oxide - toxicity; Nitric Oxide Donors - pharmacology; Nitric Oxide Donors - toxicity; Nitroso Compounds - pharmacology; oxyhemoglobin; Oxyhemoglobins - pharmacology; peroxynitrite; Rats; Superoxide Dismutase - pharmacology; Superoxides - pharmacology; Superoxides - toxicity; uric acid; Uric Acid - pharmacology; Xanthine Oxidase - metabolism
• ISSN: 1089-8603; 1089-8611
• DOI: 10.1006/niox.1999.0239

The survival of skeletal muscle myoblasts in culture after exposure either to a donor of NO, sodium nitroprusside (SNP), or ethanamine, 2,2′-(hydroxynitrosohydrazono)bis-(DETA NONOate), or to a donor of both NO and O−2, 3-morpholinosydnonimine hydrochloride (SIN-1), was investigated. SIN-1 reduced clonogenic survival markedly but donors of NO alone did not. The injurious effect of SIN-1 was prevented by oxyhemoglobin or by uric acid but not by superoxide dismutase. The exposure of myoblasts to authentic peroxynitrite (ONOO−) or to DETA NONOate in the presence of an O−2-generating system did not reduce their survival. The results show that NO or ONOO− alone is not detrimental to myoblast survival and suggest that SIN-1 toxicity is, at least in part, mediated by H2O2 in this myoblast culture system.