(ECTO)NUCLEOTIDASE KINETICS OBSERVED BY31P-NMR SPECTROSCOPY: RESOLUTION OF SIGNALS

We used31P-NMR to study the action of the drug ouabain, a digitalis glycoside, on the ecto-nucleotidase catalysed reaction pathway ATP ⇒ ADP + Pi⇒ AMP + Pi⇒ Adenosine + Pi. Even under conditions where it was impossible to resolve all resonances from the individual species, the rate constants for the...

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Published in:Pharmacological research 1997-11, Vol.36 (5), p.353-361
Main Authors: BERNARDI, MARINA, GALEAZZI, GERARDO, LAMURA, ELENA, PIANTELLI, FRANCESCO, RENDELL, JOHN, ROSSINI, LUIGI, ROSSINI, PAOLA
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Language:English
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31P-NMR spectroscopy
amphibian heart
ecto-nucleotidase kinetics
ouabain
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container_end_page 361
container_issue 5
container_start_page 353
container_title Pharmacological research
container_volume 36
creator BERNARDI, MARINA
GALEAZZI, GERARDO
LAMURA, ELENA
PIANTELLI, FRANCESCO
RENDELL, JOHN
ROSSINI, LUIGI
ROSSINI, PAOLA
description We used31P-NMR to study the action of the drug ouabain, a digitalis glycoside, on the ecto-nucleotidase catalysed reaction pathway ATP ⇒ ADP + Pi⇒ AMP + Pi⇒ Adenosine + Pi. Even under conditions where it was impossible to resolve all resonances from the individual species, the rate constants for the individual steps may be evaluated by a χl2fit of equations describing the global kinetics to the NMR data. The fitted values of the reaction rate constants show no inhibition of any step in the reaction pathway, confirming previous results. This improved kinetic method can be used to evaluate the effect of different substrates, ions and drugs, both in intact or pathologically altered organs and in tissue subcellular fractions
doi_str_mv 10.1006/phrs.1997.0189
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subjects 31P-NMR spectroscopy
amphibian heart
ecto-nucleotidase kinetics
ouabain
title (ECTO)NUCLEOTIDASE KINETICS OBSERVED BY31P-NMR SPECTROSCOPY: RESOLUTION OF SIGNALS
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