A Procedure for the Large-Scale Isolation of Major Bovine Seminal Plasma Proteins

A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a...

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Bibliographic Details
Published in:Protein expression and purification 1996-08, Vol.8 (1), p.48-56
Main Authors: Calvete, Juan J., Varela, Paloma F., Sanz, Libia, Romero, Antonio, Mann, Karlheinz, Töpfer-Petersen, Edda
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cattle
Chromatography, Affinity
Chromatography, Agarose
Chromatography, Gel
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Prostatic Secretory Proteins
Proteins - chemistry
Proteins - isolation & purification
Ribonucleases - isolation & purification
Semen - chemistry
Seminal Plasma Proteins
Tissue Inhibitor of Metalloproteinase-2
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Summary:A method for the stepwise purification of bovine seminal plasma proteins aSFP, PDC-109, TIMP-2, RNAse, and BSP-30K/PDC-109 complex is presented. It is based on the use of nondenaturing, classical chromatographic procedures. The isolation protocol is reproducible, inexpensive, easy to carry out in a common laboratory, and yields large amounts of pure proteins for biochemical and structural studies.
ISSN:1046-5928
1096-0279
DOI:10.1006/prep.1996.0073