Development of a detection system for ferric pseudobactin using monoclonal antibodies

Certain root-colonizing fluorescent pseudomonads have been shown to promote plant growth and prevent plant disease in part through the production of siderophores. However, these favorable results have not been reproduced consistently from the laboratory to the greenhouse or from the greenhouse to th...

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Bibliographic Details
Published in:Plant and soil 1991, Vol.130 (1), p.243-247
Main Authors: Buyer, J.S. (Soil Microbial Systems Lab. USDA-ARS, Beltsville, MD (USA)), Sikora, L.J, Kratzke, M.G
Format: Article
Language:English
Subjects:
Acid soils
Amino acids
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Enzyme linked immunosorbent assay
FER
HIERRO
Hybridomas
IDENTIFICACION
IDENTIFICATION
IRON
MONOCLONAL ANTIBODIES
Plant growth
Pseudomonas
PSEUDOMONAS PUTIDA
Rhizosphere
Siderophores
Soil biochemistry
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Summary:Certain root-colonizing fluorescent pseudomonads have been shown to promote plant growth and prevent plant disease in part through the production of siderophores. However, these favorable results have not been reproduced consistently from the laboratory to the greenhouse or from the greenhouse to the field. In some circumstances siderophores appear to play no role in disease prevention. In order to understand the dynamics of competition for iron in the rhizosphere it is essential that the localization and concentration of siderophores produced by both biocontrol agents and plant pathogens be determined. We have produced monoclonal antibodies (MAbs) to ferric pseudobactin, the siderophore of plant growth-promoting Pseudomonas B10. Three IgGl MAbs cross-react with certain ferric pseudobactins but not with others. A competitive ELISA has been developed to detect and quantify ferric pseudobactin.
ISSN:0032-079X
1573-5036
DOI:10.1007/BF00011879