Investigations of boron uptake at the cellular level

The nature of expression of the tolerance of barley to high levels of B at the cellular level was investigated with a view to identifying ways by which this level of expression might be exploited in a breeding programme. Using protoplasts derived from leaf tissue, it was found that genetic differenc...

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Bibliographic Details
Published in:Plant and soil 1993-10, Vol.155 (1), p.143-146
Main Authors: Jenkin, M. (Waite Agricultural Research Inst., Glen Osmond (Australia). Dept. of Plant Science), Hu, H, Brown, P, Graham, R, Lance, R, Sparrow, D
Format: Article
Language:English
Subjects:
ABSORCION DE SUSTANCIAS NUTRITIVAS
ABSORPTION DE SUBSTANCES NUTRITIVES
Barley
BORE
BORO
BORON
Cell walls
Chemical suspensions
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Genotypes
Germination
HORDEUM VULGARE
NUTRIENT UPTAKE
Plants
POLEN
POLLEN
Pollen germination
PROTOPLASTE
PROTOPLASTOS
PROTOPLASTS
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Summary:The nature of expression of the tolerance of barley to high levels of B at the cellular level was investigated with a view to identifying ways by which this level of expression might be exploited in a breeding programme. Using protoplasts derived from leaf tissue, it was found that genetic differences between B tolerant and intolerant barleys were not expressed in the absence of cell walls. Barley genotypes differing in their tolerance to B were subjected to high levels of B in the growth medium from pollen formation onwards. The genetic distribution of segregating populations in the next generation was not changed for tolerance to high B. Results also suggested that genetic tolerance to B is expressed by pollen in vitro.
ISSN:0032-079X
1573-5036
DOI:10.1007/BF00025004