Activation of the alternative complement pathway and generation of stimulating factors for granulocytes by glass fibers

Continuous-filament glass fibers coated with organic agents, candidate asbestos substitutes, were assessed for their ability to elicit from normal human serum complement-derived cleavage products which are able to stimulate the chemotaxis and the respiratory burst of polymorphonuclear leukocytes. Gl...

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Bibliographic Details
Published in:Cell biology and toxicology 1988-06, Vol.4 (2), p.187-197
Main Authors: Governa, M, Valentino, M, Visona, I, Marchiseppe, I, Lo Martire, N
Format: Article
Language:English
Subjects:
Asbestos - pharmacology
Chemotactic Factors - physiology
Chemotaxis
Complement Activation - drug effects
Complement Pathway, Alternative - drug effects
Glass
Granulocytes
Humans
Neutrophils - physiology
Superoxides - metabolism
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Summary:Continuous-filament glass fibers coated with organic agents, candidate asbestos substitutes, were assessed for their ability to elicit from normal human serum complement-derived cleavage products which are able to stimulate the chemotaxis and the respiratory burst of polymorphonuclear leukocytes. Glass fibers generated chemoattracting and respiratory stimulating factors for polymorphonuclears from human serum. The effect was dose related for chemotaxis from the serum fiber concentration of 75 micrograms/ml to 1,250 micrograms/ml. The serum chemoattracting activity, as well the respiratory stimulation, were dramatically impaired when serum had been preliminarily absorbed with antiC5 antiserum. Since the impairment of chemotactic activity occurred also in the presence of EDTA, but not in the presence of EGTA, we assumed an activation of the alternative complement pathway. Glass fibers were studied in comparison to a UICC sample of Canadian chrysotile asbestos, which is able to activate in vitro the alternative complement pathway. Glass fibers exhibited less ability than asbestos fibers to generate complement cleavage products with chemotactic activity for polymorphonuclears; however, they produced an activity about equal to 80% of a chemotactic standard stimulus such as zymosan-activated plasma.
ISSN:0742-2091
1573-6822
DOI:10.1007/BF00119245