Construction of a new strain of Streptomyces violaceoniger, having strong, constitutive and stable glucose-isomerase activity

A newly isolated strong streptomyces promoter (P1) has been cloned in front of the xylA gene of Streptomyces violaceoniger. This led to a strong and constitutive expression. To avoid instability of plasmid and glucose isomerase activity, the P1-xylA gene has been integrated into the chromosome using...

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Bibliographic Details
Published in:Biotechnology letters 1994-12, Vol.16 (12), p.1259-1264
Main Authors: Bejar, S, Belghith, K, Gargouri, R, Ellouz, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cloning
enzyme activity
Fundamental and applied biological sciences. Psychology
gene expression
genes
Genetic engineering
Genetic technics
glucose
isomerases
Methods. Procedures. Technologies
Modification of gene expression level
p1-xyla gene
plasmids
promoter regions
Streptomyces
xyla gene
xylose
xylose isomerase
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Summary:A newly isolated strong streptomyces promoter (P1) has been cloned in front of the xylA gene of Streptomyces violaceoniger. This led to a strong and constitutive expression. To avoid instability of plasmid and glucose isomerase activity, the P1-xylA gene has been integrated into the chromosome using the integrative vector pTS55. The resultant CBS1 strain has about seven times higher glucose-isomerase activity in absence of xylose compared to that of wild type strain fully induced by xylose. In addition, glucose isomerase specific activity of the CBS1 strain increases in the secondary growth phase, in contrast to wild type strain.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00149628