Imaging pattern of radiolabelled lymphokine-activated killer cells in patients with metastatic malignant melanoma

In patients with metastatic malignant melanoma the distribution patterns of radiolabelled lymphokine-activated killer (LAK) cells were investigated. Peripheral mononuclear cells (PMC) were isolated from six patients. LAK cells were generated by culturing PMC in complete medium containing 1000 U inte...

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Bibliographic Details
Published in:European Journal of Nuclear Medicine 1991-02, Vol.18 (2), p.106-110
Main Authors: SCHÄFER, E, DUMMER, R, EILLES, C, BÖRNER, W, MARTIN, R, RENDL, J, BURG, G
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Humans
Immunotherapy, Adoptive
Indium Radioisotopes
Interleukin-2 - immunology
Investigative techniques, diagnostic techniques (general aspects)
Isotope Labeling
Killer Cells, Lymphokine-Activated - transplantation
Male
Medical sciences
Melanoma - diagnostic imaging
Melanoma - secondary
Melanoma - therapy
Middle Aged
Miscellaneous. Technology
Radionuclide Imaging
Radionuclide investigations
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Summary:In patients with metastatic malignant melanoma the distribution patterns of radiolabelled lymphokine-activated killer (LAK) cells were investigated. Peripheral mononuclear cells (PMC) were isolated from six patients. LAK cells were generated by culturing PMC in complete medium containing 1000 U interleukin (IL)-2/ml and labelled with indium 111 before retransfer. We obtained scans at 2.5, 24, 48 or 96 h after injection with a high resolution gamma-camera. Intravenously injected LAK cells distributed to the lungs, liver, spleen and bone marrow. External tumour detection of known lymph node and bone metastases was successful in four. It failed in one patient with a solitary lung metastasis and in another patient with subcutaneous metastases. Our results suggest that LAK cells show tumour homing, providing a direct interaction between tumour and cytotoxic cells. We conclude that PMC seem to retain their ability to migrate after IL-2 stimulation and 111In-labeling. This technique may be helpful for kinetics studies or external detection of metastases in patients with malignant melanoma.
ISSN:0340-6997
1619-7089
DOI:10.1007/BF00175913