Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme

We have isolated 4 cDNA clones (GRT1-4) encoding glutathione reductase (GR) from a tobacco (Nicotiana tabacum L.) leaf cDNA library. The cDNAs were almost identical: GRT1, GRT3 and GRT4 represented the same gene, differing only in that GRT4 contained an intron within the C-terminal part of the codin...

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Bibliographic Details
Published in:Planta 1995, Vol.197 (2), p.422-425
Main Authors: Creissen, G.P, Mullineaux, P.M. (Norwich Research Park (United Kingdom). John Innes Centre, Dept. of Applied Genetics)
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Amino acids
Aminosaeuresequenz
ARN
Base Sequence
Biological and medical sciences
Chloroplasts
Cloning, Molecular
Complementary DNA
DNA
DNA Primers
DNA, Complementary
DNA, Plant
ENZIMAS
ENZYME
ENZYMES
EXPRESION GENICA
EXPRESSION DES GENES
FEUILLE
Fundamental and applied biological sciences. Psychology
GENE
GENE EXPRESSION
GENES
Genes, Plant
Genes. Genome
Genfamilie
GLUTATHION PEROXYDASE
GLUTATHIONE PEROXIDASE
Glutathione Reductase - genetics
Glutathionreduktase
GLUTATION PEROXIDASA
HOJAS
Introns
LEAVES
Messenger RNA
Metabolism
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nicotiana - enzymology
Nicotiana - genetics
NICOTIANA TABACUM
NUCLEOTIDE SEQUENCE
Peas
PEPTIDE
PEPTIDES
PEPTIDOS
Pflanzenbau
Pflanzenphysiologie
Plant physiology and development
Plants
Plants, Toxic
Polypeptid
Protein isoforms
PROTEINAS
PROTEINE
PROTEINS
RNA
SECUENCIA NUCLEOTIDICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Splicing
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Description
Summary:We have isolated 4 cDNA clones (GRT1-4) encoding glutathione reductase (GR) from a tobacco (Nicotiana tabacum L.) leaf cDNA library. The cDNAs were almost identical: GRT1, GRT3 and GRT4 represented the same gene, differing only in that GRT4 contained an intron within the C-terminal part of the coding sequence. Failure to splice out this intron resulted in a substitution of the final 13 amino acids of the deduced amino acid sequence. A second gene was represented by GRT2. Southern blots indicated that there were two related GR genes in tobacco. The presence of multiple isoforms of GR in tobacco may be explained in part by the expression of a small gene family. In addition, alternative isoforms may result from translation of different mRNAs derived from the same gene by intron skipping during the splicing of nascent GR mRNAs.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00202667