Analytical characterization of peptide contaminants of L-tryptophan

Reversed-phase liquid chromatographic fractions of extracts of 2 preparations of eosinophilia-myalgia syndrome (EMS)-associated L-tryptophan were analyzed by proton nuclear magnetic resonance spectrometry, mass spectrometry, microbial-growth inhibition, and amino acid residue analyses. Fraction comp...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1996, Vol.30 (1), p.142-148
Main Authors: BARNHART, E. R, MAGGIO, V. L, ALEXANDER, L. R, REILLY, M. C, GELBAUM, L. T, TURNER, W. E, HINE, T. K, NEEDHAM, L. L
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Anti-Bacterial Agents - analysis
Bacillus - metabolism
Bacitracin - analysis
Biological and medical sciences
Biotechnology
Chromatography, High Pressure Liquid
Drug Contamination
Eosinophilia-Myalgia Syndrome - chemically induced
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Humans
Industrial applications and implications. Economical aspects
Magnetic Resonance Spectroscopy
Mass Spectrometry
Other active biomolecules
Production of active biomolecules
Spectrometry, Mass, Fast Atom Bombardment
Tryptophan - adverse effects
Tryptophan - biosynthesis
Tryptophan - chemistry
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Summary:Reversed-phase liquid chromatographic fractions of extracts of 2 preparations of eosinophilia-myalgia syndrome (EMS)-associated L-tryptophan were analyzed by proton nuclear magnetic resonance spectrometry, mass spectrometry, microbial-growth inhibition, and amino acid residue analyses. Fraction components demonstrated properties of an antibiotic peptide resembling bacitracin. Many peptide antibiotics like bacitracin are secondary metabolites of Bacillus species, genus of the tryptophan producer organism for the implicated manufacturer. In order to determine whether a correlation exists between individual EMS cases and the concentration of peptides or bacitracin consumed, reliable methods must be developed for quantification of the total of isoforms.
ISSN:0090-4341
1432-0703
DOI:10.1007/BF00211341