Chicken egg yolk antibodies for cytokinin analysis

The production, isolation, and purification of specific chicken immunoglobulins (Igs) against three main groups of naturally occurring cytokinins are reported. The specific Igs directed against, respectively, zeatin riboside, dihydrozeatin riboside, and isopentenyladenosine are extracted from the eg...

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Bibliographic Details
Published in:Journal of plant growth regulation 1996, Vol.15 (1), p.19-25
Main Authors: Redig, P. (University of Antwerp, Antwerpen, Belgium.), Prinsen, E, Schryvers, N, Onckelen, H. van
Format: Article
Language:English
Subjects:
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
Analytical, structural and metabolic biochemistry
ANTICORPS
ANTICUERPOS
Biological and medical sciences
CITOQUININAS
CYTOKININE
EXTRACTOS VEGETALES
EXTRAIT D'ORIGINE VEGETALE
FISIOLOGIA VEGETAL
Fundamental and applied biological sciences. Psychology
Growth regulators
IMMUNOGLOBULINE
INMUNOGLOBULINA
JAUNE D'OEUF
Metabolism
METHODE
METODOS
Other biological molecules
PHYSIOLOGIE VEGETALE
Plant growth substances
Plant physiology and development
POLLO
POULET
PURIFICACION
PURIFICATION
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
TECHNIQUE RADIOIMMUNOLOGIQUE
TECNICAS RADIOINMUNOLOGICAS
YEMA DE HUEVO
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Summary:The production, isolation, and purification of specific chicken immunoglobulins (Igs) against three main groups of naturally occurring cytokinins are reported. The specific Igs directed against, respectively, zeatin riboside, dihydrozeatin riboside, and isopentenyladenosine are extracted from the egg yolk and used in radioimmunoassays that allow the quantification in parallel of pmol of the cytokinins in plant extracts. As little as 50 fmol of zeatin riboside, 20 fmol of isopentenyladenosine, and 40 fmol of dihydrozeatin riboside can be detected. The levels of cytokinins measured in the radioimmunoassay correlate well with physicochemical analysis methods such as high performance liquid chromatography (HPLC) with UV spectrum detection and HPLC-coupled mass spectrometric detection. Cross-reactivity studies indicate that the assay is not affected by most of the structurally related compounds. The respective antibody preparations recognized zeatin riboside, dihydrozeatin riboside, and isopentenyladenosine and the corresponding free bases. The results obtained when analyzing crude plant extracts are expressed as zeatin riboside equivalents, dihydrozeatin riboside equivalents, and isopentenyladenosine equivalents
ISSN:0721-7595
1435-8107
DOI:10.1007/BF00213130