Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on the chromosome-1 of B. patellaris and B. procumbens

New members of a satellite DNA family (Sat121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera s...

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Bibliographic Details
Published in:Theoretical and applied genetics 1994-10, Vol.89 (4), p.459-466
Main Authors: Salentijn, E.M.J, Sandal, N.N, Klein-Lankhorst, R, Lange, W, Bock, T.S.M. de, Marcker, K.A, Stiekema, W.J
Format: Article
Language:English
Subjects:
addition lines
Beta
beta patellaris
Beta procumbens
Beta vulgaris subsp. vulgaris
Biological and medical sciences
chromosome addition
chromosome fragment addition
chromosome fragments
chromosomes
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Heterodera schachtii
hs1 locus
introgression
loci
molecular sequence data
nucleotide sequences
pest resistance
Pteridophyta, spermatophyta
pulsed field electrophoresis
repetitive sequences
satellite DNA
Southern blotting
Vegetals
wild plants
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Summary:New members of a satellite DNA family (Sat121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1)in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1Pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat121 clusters were detected near the Hs1pro-1 locus.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00225381