Light-induced membrane potential changes of epidermal and mesophyll cells in growing leaves of Pisum sativum

Light transiently depolarizes the membrane of growing leaf cells. The ionic basis for changes in cell membrane electrical potentials in response to light has been determined separately for growing epidermal and mesophyll cells of the argenteum mutant of pea (Pisum sativum L.). In mesophyll cells lig...

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Bibliographic Details
Published in:Planta 1995-08, Vol.197 (1), p.127-134
Main Authors: Elzenga, J.T.M, Prins, H.B.A, Volkenburgh, E. van (Washington Univ., Seattle (USA). Dept. of Botany)
Format: Article
Language:English
Subjects:
ATPASA
ATPASE
Biological and medical sciences
CALCIO
CALCIUM
Cell membranes
Cell physiology
CELLS
CELLULE
CELULAS
CHLORIDES
CHLORURE
CLORUROS
Depolarisation
Depolarization
ELECTRIC POTENTIAL
Epidermal cells
EPIDERME
EPIDERMIS
FEUILLE
FOTOSINTESIS
Fundamental and applied biological sciences. Psychology
Gemuesebau
HOJAS
ION
IONES
IONS
LEAVES
LIGHT
LUMIERE
LUZ
Membrane potential
Membranpotential
MESOFILO
MESOPHYLL
Mesophyll cells
MESOPHYLLE
MUTANT
MUTANTES
MUTANTS
Peas
Pflanzenphysiologie
PHOTOSYNTHESE
PHOTOSYNTHESIS
Pisum
PISUM SATIVUM
Plant cells
Plant physiology and development
Plants
Plasma membrane and permeation
POTASIO
POTASSIUM
POTENCIAL ELECTRICO
POTENTIEL ELECTRIQUE
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Summary:Light transiently depolarizes the membrane of growing leaf cells. The ionic basis for changes in cell membrane electrical potentials in response to light has been determined separately for growing epidermal and mesophyll cells of the argenteum mutant of pea (Pisum sativum L.). In mesophyll cells light induces a large, transient depolarization that depends on the external Cl- concentration, is unaffected by changes in the external Ca2+ or K+ concentration, is stimulated by K+-channel blockers tetraethylammonium (TEA+) and Ba2+, and is inhibited by 3-(3′-4′-dichlorophenyl)-1,1-dimethylurea (DCMU). In isolated epidermal tissue, light induces a small, transient depolarization followed by a hyperpolarization of the membrane potential. The depolarization is enhanced by increasing the external Ca2+ concentration and by addition of Ba2+, and is not sensitive to DCMU. Epidermal cells in contact with mesophyll display a depolarization resembling the response of the underlying mesophyll cells. The light-induced depolarization in mesophyll cells seems to be mediated by an increased efflux of Cl- while the membrane-potential changes in epidermal strips reflect changes in the fluxes of Ca2+ and in the activity of the proton-pumping ATPase.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00239948