Muscle fiber typing in routinely processed skeletal muscle with monoclonal antibodies

Muscle fiber typing is conventionally performed using mATPase enzyme histochemistry on cryostat sections. After pre-incubation of sections at pH 4.3, 4.6 and 10.3, based on the pattern of enzyme reactivity, the fibers can be classified in types I, II (subtypes A, AB and B) and the intermediate C (I...

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Bibliographic Details
Published in:Histochemistry 1990-03, Vol.93 (5), p.497-499
Main Authors: Havenith, M G, Visser, R, Schrijvers-van Schendel, J M, Bosman, F T
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Humans
Hydrogen-Ion Concentration
Immunohistochemistry
Microtomy
Muscles - analysis
Muscles - ultrastructure
Myosins - analysis
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Summary:Muscle fiber typing is conventionally performed using mATPase enzyme histochemistry on cryostat sections. After pre-incubation of sections at pH 4.3, 4.6 and 10.3, based on the pattern of enzyme reactivity, the fibers can be classified in types I, II (subtypes A, AB and B) and the intermediate C (I and II) fibers. We have attempted to perform fiber typing of human psoas muscle by immunohistochemistry, using monoclonal antibodies R11D10 (specific for cardiac and type I skeletal myosin) and MY-32 (specific for fast muscle fibers) on cryostat as well as on paraffin sections. Staining of consecutive cryostat sections showed that type I fibers are R11D10 reactive whereas type II fibers are MY-32 reactive. Subtyping of type II fibers could not be performed by immunohistochemistry. Quantitative analysis of type I and II fibers showed that enzyme histochemical and immunohistochemical analysis are in close agreement.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00266407