In situ hybridization of atrial natriuretic peptide mRNA in the endothelial cells of human umbilical vessels

The localization of mRNA encoding preproatrial natriuretic peptide (ANP) was investigated in cultured human umbilical vein endothelial cells (HUVEC) and tissue preparations of umbilical vein and artery. The techniques used were in situ hybridization and in situ hybridization combined with immunocyto...

Full description

Saved in:
Bibliographic Details
Published in:Histochemistry 1993-10, Vol.100 (4), p.277-283
Main Authors: WEN QIN CAI, TERENGHI, G, BODIN, P, BURNSTOCK, G, POLAK, J. M
Format: Article
Language:English
Subjects:
Atrial Natriuretic Factor - biosynthesis
Biological and medical sciences
Blood vessels and receptors
Cells, Cultured
Digoxigenin - immunology
Digoxigenin - metabolism
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Immunohistochemistry
In Situ Hybridization
Ribonucleases - immunology
Ribonucleases - metabolism
RNA Probes
RNA, Messenger - metabolism
Umbilical Arteries - cytology
Umbilical Arteries - metabolism
Umbilical Veins - cytology
Umbilical Veins - metabolism
Vertebrates: cardiovascular system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The localization of mRNA encoding preproatrial natriuretic peptide (ANP) was investigated in cultured human umbilical vein endothelial cells (HUVEC) and tissue preparations of umbilical vein and artery. The techniques used were in situ hybridization and in situ hybridization combined with immunocytochemistry, using 32P-radiolabelled and non-radioactive digoxigenin labelled complementary RNA probes. Human ANP mRNAs are mainly localized in the endothelial cells of the umbilical vein and, to a lesser extent, in the endothelial cells of the umbilical artery. The autoradiographic labelling and the intensity of digoxigenin staining were significantly reduced by treatment with RNase before in situ hybridization. This study provides unequivocal evidence for the expression of the ANP gene in the endothelial cells of human umbilical vessels, confirming that these endothelial cells have the ability to synthesize this peptide. The functional significance of the presence of the ANP mRNA in the endothelial cells of human umbilical vessels is discussed.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00270047