Expression of enhancing factor gene and its localization in mouse tissues

Enhancing factor (EF), a 14 kDa protein, isolated from mouse small intestines, has been reported from this laboratory. Based on our earlier studies EF has been implicated in cell proliferation. Preliminary immunohistochemical studies have shown EF to be localized in the Paneth cells of small intesti...

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Bibliographic Details
Published in:Histochemistry 1991-01, Vol.96 (4), p.367-370
Main Authors: MULHERKAR, R, DESAI, S. J, RAO, R. S, WAGLE, A. S, DEO, M. G
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Antibodies
Biological and medical sciences
Blotting, Northern
DNA Probes
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Group II Phospholipases A2
Immunohistochemistry
Intestine, Small - metabolism
Male
Mice
Mice, Inbred BALB C
Miscellaneous
Peptide Biosynthesis
Peptides - analysis
Peptides - genetics
Phospholipases A
Proteins
Transcription, Genetic
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Summary:Enhancing factor (EF), a 14 kDa protein, isolated from mouse small intestines, has been reported from this laboratory. Based on our earlier studies EF has been implicated in cell proliferation. Preliminary immunohistochemical studies have shown EF to be localized in the Paneth cells of small intestines. In this paper we report the tissue distribution of EF using conditions optimized for immunohistochemical staining. In addition, the data are supported by northern blot analysis using a nick translated cDNA probe specific for EF. The results indicate that EF gene is actively transcribed mainly in the intestines. The chief source of synthesis of EF appears to be the Paneth cells located at the base of the crypts of Lieberkühn.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00271358