Isolation of mammalian cell mutants deficient in glucose 6-phosphate dehydrogenase by means of a replica-plating technique

A replica-plating technique is described which was used for the isolation of G-6-PD-deficient mutants in cultures of mutagen-treated Chinese hamster cells. Mutants were recognized by their failure to stain in a histochemical G-6-PD-specific staining reaction. Four mutants were isolated and character...

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Bibliographic Details
Published in:Human genetics 1976-01, Vol.32 (3), p.315-322
Main Authors: Wild, D, Hellkuhl, B
Format: Article
Language:English
Subjects:
Animals
Cell Separation - methods
Cells, Cultured
Cricetinae
Cytogenetics
Glucosephosphate Dehydrogenase - analysis
Glucosephosphate Dehydrogenase Deficiency
Mutagens
Mutation
Staining and Labeling
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Summary:A replica-plating technique is described which was used for the isolation of G-6-PD-deficient mutants in cultures of mutagen-treated Chinese hamster cells. Mutants were recognized by their failure to stain in a histochemical G-6-PD-specific staining reaction. Four mutants were isolated and characterized by growth properties, stability of their variant phenotypes, and reduced G-6-PD activity. One of these mutants on electrophoresis exhibited a variant G-6-PD and thus is very likely the result of a mutation in the structural gene for G-6-PD.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00295822