Effect of different fixatives on Con A surface receptors of mouse peritoneal macrophages

The effects of glutaraldehyde, formaldehyde, or osmium tetroxide fixation on the number of labeled Con A surface receptors on mouse peritoneal macrophages were compared. Gold-labeled Con A receptors were found to be isolatedly arranged and evenly distributed on cell surfaces independent of the fixat...

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Bibliographic Details
Published in:Histochemistry 1990-01, Vol.93 (4), p.443-446
Main Authors: PESCHKE, T, WOLLWEBER, L, GABERT, A, AUGSTEN, K, STRACKE, E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell receptors
Cell structures and functions
Concanavalin A - analogs & derivatives
Concanavalin A - metabolism
Electron Probe Microanalysis
Fixatives - pharmacology
Fluorescein-5-isothiocyanate - analogs & derivatives
Fluoresceins - metabolism
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Macrophages - ultrastructure
Male
Mice
Microscopy, Electron
Miscellaneous
Molecular and cellular biology
Osmium Tetroxide
Peritoneal Cavity
Receptors, Concanavalin A - drug effects
Receptors, Concanavalin A - metabolism
Spectrometry, Fluorescence
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Summary:The effects of glutaraldehyde, formaldehyde, or osmium tetroxide fixation on the number of labeled Con A surface receptors on mouse peritoneal macrophages were compared. Gold-labeled Con A receptors were found to be isolatedly arranged and evenly distributed on cell surfaces independent of the fixative used. Only cells preincubated with Con A and subsequently fixed by osmium tetroxide showed arrangement of labeled receptors in clusters. Significant differences were found in the number of Con A receptors per cell depending on the fixative used. The fluorescence intensity of FITC-Con A staining was detected spectrophotometrically, the characteristic X-rays of gold-labeled Con A receptors were determined by means of electron beam-induced X-ray microanalysis. The experimental results obtained both at light and electron microscopic level pointed to formaldehyde being the best fixative also for this purpose.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00315865