Can whole blood be used instead of separated low density cells for examination of the mitogen response?

The response of whole blood and separated low density cells to 3 mitogenic lectins [Phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin (Con-A)] was tested in 133 normal individual of all ages, including cord blood. The results obtained with the two methods correlated poorly (r = 0.16...

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Bibliographic Details
Published in:Blut 1981-07, Vol.43 (1), p.41-46
Main Authors: Nissen, C, Albrecht, R, Corneo, M, Bruun, D, Speck, B
Format: Article
Language:English
Subjects:
Blood
Blood Cells - drug effects
Concanavalin A - pharmacology
Humans
Lymphocyte Activation
Mitogens - pharmacology
Phytohemagglutinins - pharmacology
Pokeweed Mitogens - pharmacology
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Summary:The response of whole blood and separated low density cells to 3 mitogenic lectins [Phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin (Con-A)] was tested in 133 normal individual of all ages, including cord blood. The results obtained with the two methods correlated poorly (r = 0.16 for PHA; 0.47 for PWM and 0.42 for Con-A). Thus, the two methods measure different things and results are not comparable.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF00319930