Isolation of a colony-stimulating factor produced by L 1210 murine leukemia cells

Murine L1210 leukemia cells spontaneously produce very low amounts of colony stimulating factor (CSF). CSF production was markedly increased by stimulating L1210 cells with lipopolysaccharide, lectins, and sheep red blood cells. From the conditioned medium of phytohemagglutinin-stimulated L1210 cell...

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Bibliographic Details
Published in:Blut 1987-12, Vol.55 (6), p.499-504
Main Authors: Pessina, A, Muschiato, A, Neri, M G
Format: Article
Language:English
Subjects:
Animals
Colony-Stimulating Factors - isolation & purification
Concanavalin A - pharmacology
Erythrocytes - immunology
Female
Leukemia L1210 - immunology
Leukemia L1210 - pathology
Lipopolysaccharides - pharmacology
Mice
Phytohemagglutinins - pharmacology
Pokeweed Mitogens - pharmacology
Sheep - immunology
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Summary:Murine L1210 leukemia cells spontaneously produce very low amounts of colony stimulating factor (CSF). CSF production was markedly increased by stimulating L1210 cells with lipopolysaccharide, lectins, and sheep red blood cells. From the conditioned medium of phytohemagglutinin-stimulated L1210 cells we isolated a CSF with an apparent molecular weight of approximately 27,000. This CSF promoted the proliferation and the differentiation of murine GM-CFU showing a weak differentiation-inducing activity on WEHI-3 D (+) cells.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF00320219