Ultrastructural analysis of membrane-bound polysomes in human myeloma cells

We prepared ultra-thin sections of human myeloma cells, in which the rER was cut tangentially, and studied the make-up and distribution of membrane-bound polysomes electromicroscopically. In IgG myeloma large and small polysomes were detected. The polysome distribution curve showed a high peak at 7...

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Bibliographic Details
Published in:Blut 1982-01, Vol.44 (1), p.51-63
Main Authors: Kitani, T, Yonezawa, T, Imanaka, T, Hiraoka, A, Nasu, T
Format: Article
Language:English
Subjects:
electron microscopy
endoplasmic reticulum
Humans
Immunoglobulin A
Immunoglobulin D
Immunoglobulin E
Immunoglobulin G
man
Microscopy, Electron
Multiple Myeloma - ultrastructure
myeloma
polyribosomes
Polyribosomes - ultrastructure
ultrastructure
Waldenstrom Macroglobulinemia - pathology
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Summary:We prepared ultra-thin sections of human myeloma cells, in which the rER was cut tangentially, and studied the make-up and distribution of membrane-bound polysomes electromicroscopically. In IgG myeloma large and small polysomes were detected. The polysome distribution curve showed a high peak at 7 ribosomes and a lower peak at 17-18 ribosomes. IgA-, IgD- and IgE myeloma, as well as macroglobulinemia, showed peaks at 7 and 13 ribosomes. BJP myeloma manifested a sharp peak only at 7 ribosomes. Our results suggest that BJP myeloma has only small polysomes participating in L-chain synthesis, while the other myelomas exhibited large and small polysomes participating in H- and L-chain synthesis, respectively. The quantitative ratio of small and large polysomes was determined on the basis of an analytically corrected direct count.
ISSN:0006-5242
1432-0584
DOI:10.1007/bf00320686