Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence

A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared s...

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Bibliographic Details
Published in:Blut 1987-11, Vol.55 (5), p.459-466
Main Authors: RENNER, D, PROPP, H, QUEISSER, W
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bone Marrow Cells
Diploidy
DNA - metabolism
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Megakaryocytes - metabolism
Megakaryocytes - physiology
Megakaryocytes - ultrastructure
Ploidies
Staining and Labeling
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
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Summary:A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared several monoclonal (anti-GP IIIa and HD 19) and polyclonal (A225, RAHPS) antiplatelet antibodies for immunofluorescent staining. Among the identified cells, a small number of cells showing a diploid and tetraploid DNA content were found which must be regarded as promegakaryoblasts, representing 2.5-4.7% of all megakaryocytes. The heterogenous morphology of these precursors in panoptically stained smears is described.
ISSN:0006-5242
1432-0584
DOI:10.1007/BF00367464