Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts

A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked with...

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Bibliographic Details
Published in:Pflügers Archiv 1995-08, Vol.430 (4), p.596-598
Main Authors: Gáspár, Jr, R, Weidema, A F, Krasznai, Z, Nijweide, P J, Ypey, D L
Format: Article
Language:English
Subjects:
Animals
Chick Embryo
Culture Media
In Vitro Techniques
Osteoclasts - drug effects
Osteoclasts - metabolism
Patch-Clamp Techniques
Sodium - metabolism
Sodium Channels - drug effects
Sodium Channels - metabolism
Tetrodotoxin - pharmacology
Verapamil - pharmacology
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Summary:A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 < 15 nM) but not by verapamil (< or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00373899