Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts

A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked with...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 1995-08, Vol.430 (4), p.596-598
Main Authors: Gáspár, Jr, R, Weidema, A F, Krasznai, Z, Nijweide, P J, Ypey, D L
Format: Article
Language:English
Subjects:
Animals
Chick Embryo
Culture Media
In Vitro Techniques
Osteoclasts - drug effects
Osteoclasts - metabolism
Patch-Clamp Techniques
Sodium - metabolism
Sodium Channels - drug effects
Sodium Channels - metabolism
Tetrodotoxin - pharmacology
Verapamil - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3
cites cdi_FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3
container_end_page 598
container_issue 4
container_start_page 596
container_title Pflügers Archiv
container_volume 430
creator Gáspár, Jr, R
Weidema, A F
Krasznai, Z
Nijweide, P J
Ypey, D L
description A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 < 15 nM) but not by verapamil (< or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.
doi_str_mv 10.1007/BF00373899
format article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF00373899</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>7491289</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3</originalsourceid><addsrcrecordid>eNpFkE1LAzEURYModaxu3AtZK6MvHzNJllqsFYpu6npIMm8w2s6UJBX776206OrC5XDhHkIuGdwyAHX3MAUQSmhjjkjBpOAlByaOSbGrWVmrWp-Ss5Q-AIBLzUdkpKRhXJuCzBaY49AOefgOfZmwTyGHL6SdTZm-2BvqNzFin2noKa5c3A598NS_B_-JPR1SxsEvd2w6JyedXSa8OOSYvE0fF5NZOX99ep7cz0vPNc-lr-q6FcqgZ5UG1YFzWPHKdEJAJbxhmjFlOAJ33HBnrWwBrZMSDZdOtGJMrve7Pg4pReyadQwrG7cNg-bXRvNvYwdf7eH1xq2w_UMP98UPOj5Z2Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts</title><source>Springer LINK Archives</source><creator>Gáspár, Jr, R ; Weidema, A F ; Krasznai, Z ; Nijweide, P J ; Ypey, D L</creator><creatorcontrib>Gáspár, Jr, R ; Weidema, A F ; Krasznai, Z ; Nijweide, P J ; Ypey, D L</creatorcontrib><description>A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 &lt; 15 nM) but not by verapamil (&lt; or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/BF00373899</identifier><identifier>PMID: 7491289</identifier><language>eng</language><publisher>Germany</publisher><subject>Animals ; Chick Embryo ; Culture Media ; In Vitro Techniques ; Osteoclasts - drug effects ; Osteoclasts - metabolism ; Patch-Clamp Techniques ; Sodium - metabolism ; Sodium Channels - drug effects ; Sodium Channels - metabolism ; Tetrodotoxin - pharmacology ; Verapamil - pharmacology</subject><ispartof>Pflügers Archiv, 1995-08, Vol.430 (4), p.596-598</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3</citedby><cites>FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7491289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gáspár, Jr, R</creatorcontrib><creatorcontrib>Weidema, A F</creatorcontrib><creatorcontrib>Krasznai, Z</creatorcontrib><creatorcontrib>Nijweide, P J</creatorcontrib><creatorcontrib>Ypey, D L</creatorcontrib><title>Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch</addtitle><description>A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 &lt; 15 nM) but not by verapamil (&lt; or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.</description><subject>Animals</subject><subject>Chick Embryo</subject><subject>Culture Media</subject><subject>In Vitro Techniques</subject><subject>Osteoclasts - drug effects</subject><subject>Osteoclasts - metabolism</subject><subject>Patch-Clamp Techniques</subject><subject>Sodium - metabolism</subject><subject>Sodium Channels - drug effects</subject><subject>Sodium Channels - metabolism</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Verapamil - pharmacology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEURYModaxu3AtZK6MvHzNJllqsFYpu6npIMm8w2s6UJBX776206OrC5XDhHkIuGdwyAHX3MAUQSmhjjkjBpOAlByaOSbGrWVmrWp-Ss5Q-AIBLzUdkpKRhXJuCzBaY49AOefgOfZmwTyGHL6SdTZm-2BvqNzFin2noKa5c3A598NS_B_-JPR1SxsEvd2w6JyedXSa8OOSYvE0fF5NZOX99ep7cz0vPNc-lr-q6FcqgZ5UG1YFzWPHKdEJAJbxhmjFlOAJ33HBnrWwBrZMSDZdOtGJMrve7Pg4pReyadQwrG7cNg-bXRvNvYwdf7eH1xq2w_UMP98UPOj5Z2Q</recordid><startdate>199508</startdate><enddate>199508</enddate><creator>Gáspár, Jr, R</creator><creator>Weidema, A F</creator><creator>Krasznai, Z</creator><creator>Nijweide, P J</creator><creator>Ypey, D L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199508</creationdate><title>Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts</title><author>Gáspár, Jr, R ; Weidema, A F ; Krasznai, Z ; Nijweide, P J ; Ypey, D L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Chick Embryo</topic><topic>Culture Media</topic><topic>In Vitro Techniques</topic><topic>Osteoclasts - drug effects</topic><topic>Osteoclasts - metabolism</topic><topic>Patch-Clamp Techniques</topic><topic>Sodium - metabolism</topic><topic>Sodium Channels - drug effects</topic><topic>Sodium Channels - metabolism</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Verapamil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gáspár, Jr, R</creatorcontrib><creatorcontrib>Weidema, A F</creatorcontrib><creatorcontrib>Krasznai, Z</creatorcontrib><creatorcontrib>Nijweide, P J</creatorcontrib><creatorcontrib>Ypey, D L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gáspár, Jr, R</au><au>Weidema, A F</au><au>Krasznai, Z</au><au>Nijweide, P J</au><au>Ypey, D L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts</atitle><jtitle>Pflügers Archiv</jtitle><addtitle>Pflugers Arch</addtitle><date>1995-08</date><risdate>1995</risdate><volume>430</volume><issue>4</issue><spage>596</spage><epage>598</epage><pages>596-598</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>A voltage-dependent, fast, transient inward current was characterized in embryonic chicken osteoclasts using the permeabilized patch configuration of the patch-clamp technique. The current was activated by depolarizations to higher than -28 +/- 4 mV from a holding potential of -80 mV. It peaked within 1-1.5 ms, and inactivated within 3.3-6.9 ms. The 50% inactivation voltage was -59 +/- 6 mV with a steepness factor of 0.11 +/- 0.06. The current disappeared with the removal of extracellular Na+ and was reversibly blocked by tetrodotoxin (K0.5 &lt; 15 nM) but not by verapamil (&lt; or = 100 microM). We conclude that this new current in embryonic chicken osteoclasts is a sodium current known from excitable cells.</abstract><cop>Germany</cop><pmid>7491289</pmid><doi>10.1007/BF00373899</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0031-6768
ispartof Pflügers Archiv, 1995-08, Vol.430 (4), p.596-598
issn 0031-6768
1432-2013
language eng
recordid cdi_crossref_primary_10_1007_BF00373899
source Springer LINK Archives
subjects Animals
Chick Embryo
Culture Media
In Vitro Techniques
Osteoclasts - drug effects
Osteoclasts - metabolism
Patch-Clamp Techniques
Sodium - metabolism
Sodium Channels - drug effects
Sodium Channels - metabolism
Tetrodotoxin - pharmacology
Verapamil - pharmacology
title Tetrodotoxin-sensitive fast Na+ current in embryonic chicken osteoclasts
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T08%3A18%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tetrodotoxin-sensitive%20fast%20Na+%20current%20in%20embryonic%20chicken%20osteoclasts&rft.jtitle=Pfl%C3%BCgers%20Archiv&rft.au=G%C3%A1sp%C3%A1r,%20Jr,%20R&rft.date=1995-08&rft.volume=430&rft.issue=4&rft.spage=596&rft.epage=598&rft.pages=596-598&rft.issn=0031-6768&rft.eissn=1432-2013&rft_id=info:doi/10.1007/BF00373899&rft_dat=%3Cpubmed_cross%3E7491289%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c282t-c566d379ec15807f0bbe5259f33053c91811792e02b292baa4d0eab44e924b3d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/7491289&rfr_iscdi=true