Optimization of the biotransformation of L-tyrosine into L-dihydroxyphenylalanine (DOPA) by alginate-entrapped cells of Mucuna pruriens

The optimization of the biotransformation of L-tyrosine into L-dihydroxyphenylalanine (DOPA), and of formyl-tyrosine into formyl-DOPA by alginate-entrapped cells of Mucuna pruriens is reported. This optimization is discussed in terms of parameters that are relevant for the entrapped cell system (cha...

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Bibliographic Details
Published in:Planta 1985-11, Vol.166 (3), p.421-428
Main Authors: Wichers, H.J., Malingré, T.M., Huizing, H.J.
Format: Article
Language:English
Subjects:
Alginates
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Biotransformation
Cell lines
Cultured cells
Exhaustion
Fundamental and applied biological sciences. Psychology
Immobilized cells
Methods. Procedures. Technologies
Oxidation
Oxygen
Plant cells
Sodium
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Summary:The optimization of the biotransformation of L-tyrosine into L-dihydroxyphenylalanine (DOPA), and of formyl-tyrosine into formyl-DOPA by alginate-entrapped cells of Mucuna pruriens is reported. This optimization is discussed in terms of parameters that are relevant for the entrapped cell system (charge of the beads with cells, bead diameter, permeation of the cells) and some parameters that are relevant for the enzymatic transformation (pH, pO2, concentration of ascorbate). The optimization experiments resulted in the description of a biotransformation system which operates at a constant redox potential. With this transformation system a transformation efficiency of 70% could be obtained, at a biotransformation-rate of 435 μmol·h-1·g-1 (DW of cells) at a substrate concentration of 19 mM.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00401182