Stimulation of peripheral blood lymphocytes with autologous tumor and serum-derived fractions from patients with laryngeal carcinomas

We investigated the in vitro stimulation of peripheral blood lymphocytes with tumor extracts and serum-derived fractions from five patients with laryngeal carcinomas. Lymphocyte cultures were propagated by Interleukin-2 or phytohemagglutinin. Sera of the same patients were fractionated and the amoun...

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Bibliographic Details
Published in:Archives of Oto-Rhino-Laryngology 1986-01, Vol.243 (5), p.309-312
Main Authors: Koldovsky, P, Koldovsky, U, Ebbers, J, Vosteen, K H
Format: Article
Language:English
Subjects:
Antigen-Antibody Complex - immunology
Combined Modality Therapy
Female
Humans
Immunotherapy - methods
Laryngeal Neoplasms - immunology
Laryngeal Neoplasms - therapy
Lymphocyte Activation
Male
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Summary:We investigated the in vitro stimulation of peripheral blood lymphocytes with tumor extracts and serum-derived fractions from five patients with laryngeal carcinomas. Lymphocyte cultures were propagated by Interleukin-2 or phytohemagglutinin. Sera of the same patients were fractionated and the amount of circulating immune complexes present was measured by a Raji cell assay. A positive lymphocyte reaction was found in only two of the five cases after stimulation with autologous tumor extract. This response was determined by an increased 3H-thymidine incorporation. The reactive lymphocytes could be restimulated by autologous serum-derived fractions, which contained high or very low levels of circulating immune complexes. A characterization of different lymphocyte subsets revealed an elimination of natural killer cells and a relative enrichment of T-helper cells during in vitro stimulation and cultivation.
ISSN:0302-9530
1434-4726
DOI:10.1007/BF00460207