Phosphatase cytochemistry with cerium as trapping agent: verification of acid phosphatase and glucose-6-phosphatase reactive sites

Lead is prevalently replaced by cerium as trapping agent in phosphatase cytochemistry to prevent non-specific precipitation. Recently, substrate specific but artefactual lead precipitates have been described in the nuclear envelope (NE) and rough endoplasmic reticulum (RER) due to a local matrix eff...

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Bibliographic Details
Published in:Histochemistry 1986-01, Vol.84 (4-6), p.329-332
Main Authors: HOEFSMIT, E. C. M, HULSTAERT, C. E, KALICHARAN, D, EESTERMANS, I. L
Format: Article
Language:English
Subjects:
acid phosphatase
Acid Phosphatase - metabolism
Animals
Biological and medical sciences
Cerium
Endoplasmic Reticulum - enzymology
Fundamental and applied biological sciences. Psychology
glucose-6-phosphatase
Glucose-6-Phosphatase - metabolism
Histocytochemistry - methods
Liver - cytology
Liver - enzymology
Liver. Bile. Biliary tracts
Lysosomes - enzymology
Macrophages - enzymology
Male
Nuclear Envelope - enzymology
Peritoneal Cavity - cytology
phosphatase
Rats
Vertebrates: digestive system
Citations: Items that this one cites
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Summary:Lead is prevalently replaced by cerium as trapping agent in phosphatase cytochemistry to prevent non-specific precipitation. Recently, substrate specific but artefactual lead precipitates have been described in the nuclear envelope (NE) and rough endoplasmic reticulum (RER) due to a local matrix effect. In the present study a verification was carried out of the localization of acid phosphatase and glucose-6-phosphatase in the NE and RER of rat peritoneal macrophages and hepatocytes respectively with cerium. It appeared that precipitates of cerium phosphate in NE and RER of peritoneal macrophages do not represent sites of acid phosphatase activity but are due to the matrix effect. However, in rat hepatocytes these organelles demonstrate true reactive sites for glucose-6-phosphatase.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00482958