Direct fluorescence localization of an endogenous N-acetyl-glucosamine-specific lectin in the thyroid gland

A sugar-binding protein, or endogenous lectin, was localized on fixed and paraffin-embedded thyroid sections by means of fluorescein-labelled neoglycoproteins. Fluorescence microscopy showed the binding of this lectin to be dependent on calcium ions and acidic pH and indicated sugar specificity for...

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Published in:Histochemistry 1988, Vol.89 (2), p.171-176
Main Authors: ALQUIER, C, MIQUELIS, R, MONSIGNY, M
Format: Article
Language:English
Subjects:
Acetylglucosamine - metabolism
Animals
Biological and medical sciences
Fluorescein-5-isothiocyanate
Fluoresceins
Fundamental and applied biological sciences. Psychology
Glucosamine - analogs & derivatives
Glycoproteins
Histocytochemistry
Humans
Mice
Microscopy, Fluorescence
Rabbits
Receptors, Immunologic - metabolism
Receptors, N-Acetylglucosamine
Swine
Thiocyanates
Thyroglobulin - metabolism
Thyroid Gland - metabolism
Vertebrates: endocrinology
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cites cdi_FETCH-LOGICAL-c312t-7313a7d3769819e09d45e739af88dcd4eae09fae2efddbf5ff0e85045ecdbec33
container_end_page 176
container_issue 2
container_start_page 171
container_title Histochemistry
container_volume 89
creator ALQUIER, C
MIQUELIS, R
MONSIGNY, M
description A sugar-binding protein, or endogenous lectin, was localized on fixed and paraffin-embedded thyroid sections by means of fluorescein-labelled neoglycoproteins. Fluorescence microscopy showed the binding of this lectin to be dependent on calcium ions and acidic pH and indicated sugar specificity for GlcNAc moieties only. In human, porcine and murine thyrocytes, specific binding was observed mainly on subcellular organelles. Conversely, in rabbit thyrocytes, specific labelling was seen mostly at the apical cell surface facing the follicular lumen. The possibility that this novel endogenous lectin is involved in the Tg metabolism is considered.
doi_str_mv 10.1007/BF00489920
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ispartof Histochemistry, 1988, Vol.89 (2), p.171-176
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1432-119X
language eng
recordid cdi_crossref_primary_10_1007_BF00489920
source Springer Online Journal Archives
subjects Acetylglucosamine - metabolism
Animals
Biological and medical sciences
Fluorescein-5-isothiocyanate
Fluoresceins
Fundamental and applied biological sciences. Psychology
Glucosamine - analogs & derivatives
Glycoproteins
Histocytochemistry
Humans
Mice
Microscopy, Fluorescence
Rabbits
Receptors, Immunologic - metabolism
Receptors, N-Acetylglucosamine
Swine
Thiocyanates
Thyroglobulin - metabolism
Thyroid Gland - metabolism
Vertebrates: endocrinology
title Direct fluorescence localization of an endogenous N-acetyl-glucosamine-specific lectin in the thyroid gland
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