Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei

Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C.2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition...

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Published in:Histochemistry 1975-01, Vol.43 (1), p.73-79
Main Authors: Unger, E, Buchwalow, I B, Hartmann, M, Kleschtschinow, W M, Raikhlin, N T
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - antagonists & inhibitors
Animals
Cell Nucleus - enzymology
Cell Nucleus - metabolism
Chromatin - metabolism
Ethanol
Formaldehyde
Histocytochemistry
Liver - enzymology
Liver - ultrastructure
Mice
Microscopy, Electron
NAD
Nicotinamide Mononucleotide
Nucleotidyltransferases - metabolism
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container_title Histochemistry
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creator Unger, E
Buchwalow, I B
Hartmann, M
Kleschtschinow, W M
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description Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C.2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition by formaldehyde/ethanol prefixation. In isolated mouse liver nuclei activity of NAD-pyrophosphorylase was found in nucleoli, in interchromatin granules, coiled bodies and strand-like structures in nucleoplasm.
doi_str_mv 10.1007/BF00490156
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subjects Adenosine Triphosphatases - antagonists & inhibitors
Animals
Cell Nucleus - enzymology
Cell Nucleus - metabolism
Chromatin - metabolism
Ethanol
Formaldehyde
Histocytochemistry
Liver - enzymology
Liver - ultrastructure
Mice
Microscopy, Electron
NAD
Nicotinamide Mononucleotide
Nucleotidyltransferases - metabolism
title Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei
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