Aldehyde bisulfite-toluidine blue (ABT) staining as a topo-optical reaction for demonstration of linear order of vicinal OH groups in biological structures

The aldehyde-bisulfite-toluidine blue reaction followed by poststaining stabilization with potassium ferricyanide (ABT) is described as a topo-optical, oriented staining reaction of the vicinal OH groups of complex carbohydrates in biological structures such as polysaccharides, glycoproteins and gly...

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Bibliographic Details
Published in:Histochemistry 1975-06, Vol.43 (4), p.333-348
Main Authors: Romhányi, G, Deák, G, Fischer, J
Format: Article
Language:English
Subjects:
Aldehydes
Amylases
Basement Membrane - analysis
Birefringence
Cellulose - analysis
Glycogen - analysis
Glycolipids - analysis
Glycoproteins - analysis
Histocytochemistry
Humans
Microscopy, Polarization
Molecular Conformation
Polysaccharides - analysis
Polysaccharides, Bacterial - analysis
Staining and Labeling
Starch - analysis
Sulfites
Tolonium Chloride
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Summary:The aldehyde-bisulfite-toluidine blue reaction followed by poststaining stabilization with potassium ferricyanide (ABT) is described as a topo-optical, oriented staining reaction of the vicinal OH groups of complex carbohydrates in biological structures such as polysaccharides, glycoproteins and glycolipids. The birefringence as induced by the oriented dye binding as a result of ABT is indicative of linear order of the vicinal OH groups and, in turn, provides information on the ultrastructural pattern of carbohydrate moieties in biological substances, which pattern is often not demonstrable by other ultrastructural methods. The possibilities of this new approach to the ultrastructural analysis of complex carbohydrates with ABT in a great number of biological substances is demonstrated and its practical value in histopathology discussed.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00490192