Development of immunohistochemical characteristics of intrafusal fibres in normal and de-efferented rat muscle spindles

Intrafusal muscle fibres in adult muscle spindles differ in their myosin composition. After selective motor denervation intrafusal muscle fibres develop mature ultrastructural characteristics. In order to evaluate the role of fusimotor innervation on the maturation of the myosin composition of intra...

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Bibliographic Details
Published in:Histochemistry 1982-01, Vol.74 (3), p.355-366
Main Authors: te Kronnie, G, Donselaar, Y, Soukup, T, Zelená, J
Format: Article
Language:English
Subjects:
Animals
Denervation
Immune Sera
Microscopy, Electron
Muscle Spindles - growth & development
Muscle Spindles - immunology
Muscle Spindles - ultrastructure
Myosins - immunology
Rats
Rats, Inbred Strains
Staining and Labeling
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Summary:Intrafusal muscle fibres in adult muscle spindles differ in their myosin composition. After selective motor denervation intrafusal muscle fibres develop mature ultrastructural characteristics. In order to evaluate the role of fusimotor innervation on the maturation of the myosin composition of intrafusal muscle fibres we have examined with immunohistochemical techniques i) the postnatal development of muscle spindles in new-born rats and in 7-21 day old rats; ii) muscle spindles in the EDL of 21-day-old rats de-efferented at birth. For the characterization of myosins in intrafusal fibres we used three myosin antisera: antipectoral myosin, antiheart myosin and antiheart myosin adsorbed with muscle powder from the soleus muscle of guinea pig. We show in this study that during development intrafusal fibres change immunoreactivity and that in the absence of motor innervation bag fibres do not fully develop the myosin characteristics of control spindles. We conclude that the maturation of bag1 and bag2 fibres apparently requires next to the inductive influence of sensory axon terminals the presence and activity of fusimotor axons.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00493435