An immunocytochemical investigation with monoclonal antibodies to somatostatin

• Published in: Histochemistry 1985-01, Vol.83 (2), p.175-180
• Main Authors: BUCHAN, A. M. J, SIKORA, L. K. J, LEVY, J. G, MCINTOSH, C. H. S, DYCK, I, BROWN, J. C
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Specificity; Apud cells. Peptide and protein hormones. Growth factors; Biological and medical sciences; Cholecystokinin - metabolism; Colon - metabolism; Fundamental and applied biological sciences. Psychology; Gastric Inhibitory Polypeptide - metabolism; Gastric Mucosa - metabolism; Gastrins - metabolism; Glucagon - metabolism; Humans; Immunologic Techniques; Intestine, Small - metabolism; Motilin - metabolism; Neurotensin - metabolism; Pancreas - metabolism; Papio; Rats; Secretin - metabolism; Somatostatin - immunology; Somatostatin - metabolism; Vertebrates: endocrinology
• ISSN: 0301-5564; 1432-119X
• DOI: 10.1007/BF00495150

Four monoclonal antibodies specific for somatostatin have been produced and characterized. These antibodies were used to assess the anatomical relationship of somatostatin-containing cells in the pancreas and gastrointestinal tract of man, baboon and rat with ten other peptide-containing endocrine cells. The peptides investigated were gastrin, cholecystokinin, motilin, secretin, neurotensin, gastric inhibitory polypeptide, gut-glucagon, pancreatic glucagon, pancreatic polypeptide and insulin. The only regions in which somatostatin cells were seen in close contact with another endocrine cell were in the pancreas and the gastric antrum. In the pancreas somatostatin cells were commonly seen in close contact with insulin, glucagon and pancreatic polypeptide cells and infrequent contact was demonstrable with the gastrin-immunoreactive cells in the antrum of both rat and man. In all other cases no evidence was obtained for a close anatomical relationship between somatostatin cells and the other enteroendocrine cells.